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KMID : 0545120040140040872
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 4 p.872 ~ p.876
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Inhibitory Effect of Bacteriophage EPS-Depolymerase on Growth of Asian Pear Blight Pathogen Erwinia pyrifoliae
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Kim IG
Lee MS/Jin TE/Hwang BK/Lee JH/Suh SC/Rhim SL
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Abstract
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The plant pathogen Erwinia pyrifoliae was infected with bacteriophage PEa1(h) which produced translucent halo plaque when grown on a lawn of pyrifoliae. To investigate the function of an exopolysaccharide (EPS)-depolymerase in the growth of E. pyrifoliae an EPSdepolymerase gene was synthesized using the PCR method and sequenced. The synthesized gene was then transferred E. pyrifoliae. The transformed E. pyrifoliae did not produce any ooze and its growth was inhibited. However the EPSdepolymerase did not appear to induce cell death. Accordingly the present results suggest that an EPS-depolymerase may effective in inhibiting the cell growth or infection of pathogen E. pyrifoliae.
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